ࡱ> SVPQRi wSbjbj.. RDbDbJ" " 87J("(P"P"P","4,H, 7777777$u9+<^@7T,+@,T,T,@7P"P" U7 5 5 5T,P"P"7 5T,7 5 5 5P"WF,R 57k707 5<@-< 5 58<B5T,T, 5T,T,T,T,T,@7@7:1T,T,T,7T,T,T,T,<T,T,T,T,T,T,T,T,T," B d: Animal Care and Use Committee For Administration Use Only Protocol No.Date Received:Committee Meeting Date:TEACHING PROTOCOL New Application Form (Teaching)  The use of animals for teaching is a privilege. Before a protocol to use animals in the classroom or a teaching laboratory exercise is approved, the instructor must show that the use of animals is justified, that the project has pedagogical merit, and that the procedure to which the animals will be subjected will be carried out humanely and in accordance with CCAC standards. Approved protocols will be valid for a period of 1 year and may be renewed (with minor revisions if required) in years 2, 3 and 4 with re-application in year 5. Please submit a signed original of the completed application to the Animal Care and Use Committee, Office of Research, 3333 University Way, Prince George BC, V2N 4Z9, and an electronic version to  HYPERLINK "mailto:acuc@unbc.ca" acuc@unbc.ca. 1. GENERAL INFORMATION Course Number and Title:Has this protocol been approved before?  FORMCHECKBOX  Yes  FORMCHECKBOX  No If yes, please provide previous protocol number: Instructor / Course Director Department/ProgramPosition/RankApplication DatePhone/Fax:EmailProposed Start Date for Current ApplicationProposed End Date for This ApplicationFinal End Date of ProjectMulti-Year Teaching Protocol ____/____ (Indicate the year this application relates to) yr /of/ yrLocation where course will take place: CCAC Category of Invasiveness:  FORMCHECKBOX  A  FORMCHECKBOX  B  FORMCHECKBOX  C  FORMCHECKBOX  D  FORMCHECKBOX  E (see Definitions in Section 10 of this document for details)  CCAC Purpose of Animal Use (PAUs):  FORMCHECKBOX  0  FORMCHECKBOX  1  FORMCHECKBOX  2  FORMCHECKBOX  3  FORMCHECKBOX  4  FORMCHECKBOX  5 (see Definitions in Section 10 of this document for details)  Declaration: I, the undersigned, will ensure that all animals used in this project will be treated and cared for in accordance with the policies and guidelines of the Canadian Council on Animal Care and the requirements of the relevant international, federal, provincial and municipal legislation. I accept responsibility for keeping the information in this application current and accurate and for notifying the Animal Care and Use Committee of any deviations from this proposal. ______________________________________________ ________________________________ Instructor / Course Director Date 2. FUNDING AND PEDAGOGICAL MERIT Funding:  FORMCHECKBOX  N/A  FORMCHECKBOX  Internal, Specify ________________  FORMCHECKBOX  External, Specify ___________________ Proposed date(s) of animal use:Before a protocol to use animals in the classroom or a teaching laboratory exercise is approved, the instructor must show that the use of animals is justified, that the project has pedagogical merit. The ACUC does not assess pedagogical merit. The course instructor must discuss the use of animals, as well as describe the learning objectives and outcomes, with their Program / Department Chair. It is the responsibility of the Program / Department Chair to assess the pedagogical merit of the activity. Has this protocol been reviewed and approved by your Program / Department Chair for pedagogical merit? Yes  FORMCHECKBOX  No  FORMCHECKBOX  This protocol has been review for pedagogical merit by a minimum of two people: Yes  FORMCHECKBOX  No  FORMCHECKBOX  _____________________________________ ________________________________ Please print Program/Department Chair Name *Signature of Program/Department Chair *By signing above, you acknowledge that you have reviewed this protocol and agree that there is pedagogical value to support using live animals in this course.  3. KEY WORD DESCRIPTION PLEASE CHECK ALL THAT APPLY GeneralProceduresAgentsSurgical  FORMCHECKBOX  Acute  FORMCHECKBOX  Behavioural Study  FORMCHECKBOX  Breeding  FORMCHECKBOX  Cell Cultures  FORMCHECKBOX  Chronic  FORMCHECKBOX  Environmental Protection  FORMCHECKBOX  Pilot Study  FORMCHECKBOX  Reinforcement/Motivation  FORMCHECKBOX  Tissue/Organ Collection  FORMCHECKBOX  Transgenic Animal  FORMCHECKBOX  Observational  FORMCHECKBOX  Wild Animals  FORMCHECKBOX  Other (please specify):   FORMCHECKBOX  Blood Sampling  FORMCHECKBOX  Euthanasia  FORMCHECKBOX  Food Deprivation  FORMCHECKBOX  Gavaging  FORMCHECKBOX  Identification/Marking  FORMCHECKBOX  Injections  FORMCHECKBOX IP  FORMCHECKBOX IV  FORMCHECKBOX IM  FORMCHECKBOX SQ  FORMCHECKBOX  Physical Restraint  FORMCHECKBOX  Special Diet  FORMCHECKBOX  Trapping/Netting  FORMCHECKBOX  Water Restriction  FORMCHECKBOX  Other (Please specify):   FORMCHECKBOX  Anaesthetics  FORMCHECKBOX  Bio-Hazardous  FORMCHECKBOX  Carcinogens  FORMCHECKBOX  Chemical  FORMCHECKBOX  Infectious  FORMCHECKBOX  Immunogenic  FORMCHECKBOX  Inflammatory  FORMCHECKBOX  Other (Please specify):  FORMCHECKBOX Cannulation  FORMCHECKBOX  Major  FORMCHECKBOX  Minor  FORMCHECKBOX  Multiple  FORMCHECKBOX  Survival  FORMCHECKBOX  Terminal  FORMCHECKBOX  Other (Please specify): 4. Description (Summary) Rationale: Describe, in a short paragraph, the overall aim of the course or section of the course using animals, and its potential benefits to the students. Describe the objectives and potential value of using animals. Summarize in point form the primary objectives of the course.  Will animals be anaesthetized? Yes  FORMCHECKBOX  No  FORMCHECKBOX  If yes, please provide complete anesthetic details: Does this activity entail any surgical manipulations? Yes  FORMCHECKBOX  No  FORMCHECKBOX  If Yes, please provide complete surgical details: Will students handle live animals? Yes  FORMCHECKBOX  No  FORMCHECKBOX How many students are (or are likely to be) enrolled in the class? What will be the ratio of students to the number of animals used?What will be the ratio of instructors to students?After the course what will happen with the animals? 5. Participants directly involved in the care and use of animals in this project NamePosition ResponsibilitiesContactContact Phone Number FORMCHECKBOX  mandatory Animal Training course  FORMCHECKBOX  Surgery and Anesthesia course, if applicable  FORMCHECKBOX  mandatory Medical Surveillance Questionnaire FORMCHECKBOX  mandatory Animal Training course  FORMCHECKBOX  Surgery and Anesthesia course, if applicable  FORMCHECKBOX  mandatory Medical Surveillance Questionnaire FORMCHECKBOX  mandatory Animal Training course  FORMCHECKBOX  Surgery and Anesthesia course, if applicable  FORMCHECKBOX  mandatory Medical Surveillance QuestionnaireDescribe additional training that each participant has received or will receive. Indicate source of this training.  6. ANIMAL INFORMATION AND HOUSING: Identify the number and species of animals used in this project Species/strainNumber per yearSupplier/SourceIndicate where the animals will be housed: Indicate where animal procedures will be conducted: If wild animals are to be used, provide the name of the agency issuing all necessary permits, permit numbers, and copies of permits.  FORMCHECKBOX  N/A  endeavors to provide an appropriate species-specific enriched environment for the maintenance of all animals during short- and long-term housing. Would you prefer to use:  FORMCHECKBOX  Set, non-varying enrichment  FORMCHECKBOX  Rotating enrichment (different materials weekly)  FORMCHECKBOX  No preference  FORMCHECKBOX  No enrichment Please indicate the reasons why environmental enrichment strategies should be withheld from the animals used in your protocol: Please select allowable enrichment:  FORMCHECKBOX  Nesting material (neslets, shredded paper)  FORMCHECKBOX  Paper houses autoclaved paper products (tubes, small boxes etc.)  FORMCHECKBOX  Plastic houses  FORMCHECKBOX  Running wheels  FORMCHECKBOX  Food treats (autoclaved seeds) Absence of a house and/or nesting material must be justified: Specific instructions regarding housing and/or enrichment:  7. REPLACEMENT, REDUCTION AND REFINEMENT Please justify the number of animals requested for each species described above, based on the objectives of the course, and indicate how the numbers were determined. Describe the characteristics of the animal species selected that justifies their use in the proposed course (consider characteristics such as body size, species, strain, data from previous studies or unique anatomic/physiological features). Non-animal alternatives should be used whenever possible. Explain briefly why any available non-animal alternatives are not suitable for meeting the objectives of this course.  What are the benefits that will be gained by the students through use of live animals that cannot be gained through use of non-animal alternative teaching resources? If a student refuses to work with animals on moral grounds, will an alternative in lieu of using animals be available (e.g., video, simulation, demonstration, etc.)?  8. PAIN, DISTRESS, ENDPOINTS and EUTHANASIA Identify any pain or distress likely to be associated with the procedures or manipulations, and describe how it will be alleviated or minimized. If animals encounter unanticipated pain and/or distress, what criteria will be used to terminate the procedure and possibly euthanize the animal(s)? Endpoints are clear criteria to define the point at which humane intervention must be implemented to prevent or relieve unnecessary pain and/or distress. Should the experimental animal acquire experimentally-induced disease, illness or life-threatening condition, select the endpoint(s) of the experiment for the animal. Indicate the possible causes of any morbidity and mortality, and the number of animals that you anticipate might be affected. Acceptable methods of Euthanasia: Please specify the method of euthanasia.  FORMCHECKBOX  Injection of diluted/buffered barbiturate*  FORMCHECKBOX  Exsanguination with anesthesia  FORMCHECKBOX  Decapitation with anesthesia, list agent/dose/route*  FORMCHECKBOX  Maceration (for fish less than 2cm in length)  FORMCHECKBOX  Cervical dislocation with anesthesia, list agent/dose/route *  FORMCHECKBOX  Overdose of inhalant anesthetics with C02  FORMCHECKBOX  Immersion or injection of buffered TMS/MS222 (Fish/Frogs)  FORMCHECKBOX  Clove oil (Fish)  FORMCHECKBOX  Other (please specify): *List agent/dose/route: Conditionally Acceptable Methods: Please specify the method of euthanasia  FORMCHECKBOX  Decapitation without anesthesia  FORMCHECKBOX  Cervical dislocation without anesthesia  FORMCHECKBOX  C02 only (Rodents)  FORMCHECKBOX  Concussion (Fish)  FORMCHECKBOX  Other (please specify):The use of any Conditionally Acceptable Method must be justified. Please explain the reason why this method of euthanasia is required:  9. Emergency Plan Provision for recovery, treatment or euthanasia of injured animals and disposal of carcasses must be specified In the event that an animal is injured during handling or manipulation, identify what actions will be taken. For example, will the animal receive medical treat or be euthanized? If an animal is euthanized, specify how the carcass will be disposed of. Contact name and position in the event of animal injuryPhone Number 10. DEFINITIONS Description of Purpose of Animal Use (PAUs) 0: Breeding Colony/Stock Animals held in breeding colonies (e.g., fish, rodents) that have not been assigned to a particular research or teaching protocol. 1: Fundamental Nature Studies Studies of a fundamental nature in sciences relating to essential structure or function (e.g., biology. psycho-biochemistry, pharmacology, physiology, etc.). Possible examples are studies designed to understand: the cellular and/or molecular basis of inflammatory reactions or basic physiological or biochemical reactions; one of the various roles played by a hormone or other compound in mammals; the behavior of species; the population dynamics of various species. 2: Medical Purposes - Studies for medical purposes, including veterinary medicine, that relate to human or animal diseases. These are studies carried out to better understand a specific disease or disorder and to possibly find therapies for it. Possible examples: development of a mouse model for a specific type of cancer or other disease; studies to determine which antibodies are the most likely to contribute positively to the therapy of a specific type of cancer; studies to determine which molecule within a particular class of compounds is the most likely to contribute to maintaining stable blood glucose levels in an animal model of diabetes. 3: Regulatory Testing - Studies for regulatory testing of products for the protection of humans, animals, or the environment. Possible examples: safety testing, regulatory toxicology, vaccine efficacy trials and testing of new therapeutic compounds. 4: Development of Products - Studies for the development of products or appliances for human or veterinary medicine. These are studies that investigate potential therapies (as determined following studies of PAU 2) for humans or animals, before regulatory testing. PAU 3 is carried out on the most promising therapies. Possible examples include studies undertaken to: investigate the role and effects of a specific drug or immunotherapy candidate for cancer; develop physical devices to assist heart function; develop artificial organs. 5: Education and training Education and training of individuals in post-secondary institutions or facilities. These are teaching or training programs where animals are used to introduce students to scientific work and teach manual skills and techniques. Category of Invasiveness* *(Excerpt from the 1991 CCAC policy statement on: Categories of Invasiveness in Animal Experiments) A: Experiments on most invertebrates or on live isolates. Possible examples are the use of tissue culture and tissues obtained at necropsy or from the slaughterhouse; the use of eggs, protozoa or other single-celled organisms; experiments involving containment, incision or other invasive procedures on metazoa. B: Experiments which cause little or no discomfort or stress. Possible examples: domestic flocks or herds being maintained in simulated or actual commercial production management systems; the short-term and skillful restraint of animals for purposes of observation or physical examination; blood sampling; injection of material in amounts that will not cause adverse reactions by the following routes: intravenous, subcutaneous, intramuscular, intraperitoneal, or oral, but not intrathoracic or intracardiac (Category C); acute non-survival studies in which the animals are completely anesthetized and do not regain consciousness prior to euthanasia; short periods of food and/or water deprivation equivalent to periods of abstinence in nature. C Experiments which cause minor stress or pain of short duration. Possible examples: cannulation or catheterization of blood vessels or body cavities under anesthesia; minor surgical procedures under anesthesia, such as biopsies, laparoscopy; short periods of restraint beyond that for simple observation or examination, but consistent with minimal distress; short periods of food and/or water deprivation which exceed periods of abstinence in nature; behavioural experiments on conscious animals that involve short-term, stressful restraint; exposure to non-lethal levels of drugs or chemicals. Such procedures should not cause significant changes in the animal's appearance, in physiological parameters such as respiratory or cardiac rate, fecal or urinary output, or in social responses. Note: During or after Category C studies, animals must not show self-mutilation, anorexia, dehydration, hyperactivity, increased recumbency or dormancy, increased vocalization, aggressive-defensive behaviour or demonstrate social withdrawal and self-isolation. D: Experiments which cause moderate to severe distress or discomfort. Possible examples: major surgical procedures conducted under general anesthesia, with subsequent recovery; prolonged (several hours or more) periods of physical restraint; induction of behavioural stresses such as maternal deprivation, aggression, predator-prey interactions; procedures which cause severe, persistent or irreversible disruption of sensorimotor organization; the use of Freund's Complete Adjuvant (FCA) (see CCAC policy statement on: acceptable immunological procedures). Other examples include induction of anatomical and physiological abnormalities that will result in pain or distress; the exposure of an animal to noxious stimuli from which escape is impossible; the production of radiation sickness; exposure to drugs or chemicals at levels that impair physiological systems. Note: Procedures used in Category D studies should not cause prolonged or severe clinical distress as may be exhibited by a wide range of clinical signs, such as marked abnormalities in behavioural patterns or attitudes, the absence of grooming, dehydration, abnormal vocalization, prolonged anorexia, circulatory collapse, extreme lethargy or disinclination to move, and clinical signs of severe or advanced local or systemic infection, etc. E: Procedures which cause severe pain near, at, or above the pain tolerance threshold of an unanesthetized conscious animal. This Category of Invasiveness is not necessarily confined to surgical procedures, but may include exposure to noxious stimuli or agents whose effects are unknown; exposure to drugs or chemicals at levels that (may) markedly impair physiological systems and which cause death, severe pain, or extreme distress; completely new biomedical experiments which have a high degree of invasiveness; behavioural studies about which the effects of the degree of distress are not known; use of muscle relaxants or paralytic drugs without anesthetics; burn or trauma infliction on unanesthetized animals; a euthanasia method not approved by the CCAC; any procedures (e.g., the injection of noxious agents or the induction of severe stress or shock) that will result in pain which approaches the pain tolerance threshold and cannot be relieved by analgesia (e.g., when toxicity testing and experimentally-induced infectious disease studies have death as the endpoint).     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